University Graduate School 2002-2004 Online Bulletin: Biochemistry

Graduate Faculty

Clyde Culbertson Professor of Biology
Carl Bauer (Biology)

Lilly Chemistry Alumni Chair
Milos Novotny (Chemistry)

Distinguished Professors
Carl Bauer (Biology), Howard Gest (Emeritus, Biology), Milos Novotny (Chemistry), Peter Ortoleva (Chemistry), Frank Putnam (Emeritus, Biology), Anthony San Pietro (Emeritus, Biology)

Professors
Peter Cherbas (Biology), David Clemmer (Chemistry), Harry Day (Emeritus, Chemistry), Thomas Donahue (Biology), W. Terry Jenkins (Emeritus, Chemistry), Arthur Koch (Emeritus, Biology), James Reilly (Chemistry, John P. Richardson (Chemistry), Milton Taylor (Biology), Robert Togasaki (Emeritus, Biology)

Associate Professors
Jose Bonner (Biology), Yves Brun (Biology), David Daleke (Biochemistry, Medical Sciences), C. Cheng Kao (Biology), Joseph Near (Pharmacology), Shuming Nie (Chemistry), Theodore Widlanski (Chemistry)

Assistant Professors
Donald Burke* (Chemistry), Lingling Chen* (Biology), Jim Drummond* (Biology), Andrew Feig* (Chemistry), John Foley* (Anatomy and Cell Biology, Medical Sciences), Clay Fuqua* (Biology), Richard Hardy* (Biology), Evelyn Jabri* (Chemistry), David Kehoe* (Biology), Ken Nephew* (Physiology Medical Sciences), Martha Oakley* (Chemistry), Martin Stone* (Chemistry), Jay Tang* (Physics), Claire Walczak* (Biochemistry, Medical Sciences), Kelly Williams* (Biology), Joel Ybe* (Biology), Jeffrey Zaleski* (Chemistry)

Graduate Advisor
Professor Carl Bauer, Myers Hall 150C, (812) 856-0192

Degrees Offered

Master of Science and Doctor of Philosophy

Special Program Requirements

(See also general University Graduate School requirements.)

Admission Requirements
Undergraduate course work must include two semesters of organic chemistry and one semester of biochemistry. Though not required, one semester of molecular biology and two semesters of biology are recommended. One semester of (bio)physical chemistry is strongly recommended. Deficiencies in required courses must be removed during the first year of graduate study.

Master of Science Degree

Course Requirements
A minimum of 30 credit hours, of which 18 credit hours must be in course work other than research, including 12 credit hours in biochemistry and 6 credit hours in basic graduate-level courses.

Thesis
Required.

Final Examination
Oral, covering thesis and major.

Doctor of Philosophy Degree

Course Requirements
A total of 90 credit hours, including 18 credit hours, satisfied by the core courses (B501, B502, B503, B504, B580), and three semesters of B600. The sequence of courses comprising the major must be approved by the student's advisory committee.

Minor
A doctoral student in biochemistry may minor in any appropriate discipline, following the requirements specified by the minor department. The sequence of courses comprising the minor must be approved by the student's advisory committee. The core Biochemistry courses (B501, B502, B503, B504) may be used to fulfill the minor requirement, but a single course may not be credited to both the major and minor requirement.

Qualifying Examinations
In the fifth semester, students meet with their examination committee to review past performance and to evaluate plans for completing the Ph.D. oral and written presentations of research progress are required.

Final Examination
Oral, covering dissertation, major, and minor.

Ph.D. Minor in Biochemistry
Students from other programs who wish to minor in biochemistry must complete at least 6 credit hours of graduate course work in biochemistry, excluding B502, B580 and B600, with an average of B (3.0) or above. At least one of the courses must be B501, B503, or B504.

Courses

Biochemistry

B501 Integrated Biochemistry (4.5 cr) P: Undergraduate biochemistry (equivalent to C483 or C484) or consent of instructor. Basic principals and methodologies of biochemistry; essentials of macromolecular biosynthesis; mechanism-based examination of biochemical aspects of cell biology; material is presented with an integrative approach designed to illustrate the inter-relationship of biochemical processes.

B502 Analysis of Biochemical Literature (1.5 cr.) P: Concurrent enrollment in B501 or consent of instructor. Critical evaluation of the biochemical literature, using selected papers as examples; development of written and oral communication skills in the context of literature analysis.

B503 Macromolecular Structure and Interaction (3 cr.) P: B501 or undergraduate biochemistry (equivalent to C483 or C484), one semester of undergraduate organic chemistry (equivalent to C341) or consent of instructor. Undergraduate (bio)physical chemistry (equivalent to C481 or C361) is strongly recommended. Principals of inter-and intra-molecular interactions; structural stability of proteins and nucleic acids; thermodynamic and kinetic analysis of complex binding; experimental methods for analysis of macromolecular structure and binding.

B504 Biomolecular Catalysis (3 cr.) P: Undergraduate organic chemistry (equivalent to C342), undergraduate biochemistry (equivalent to C483 or C484) or consent of instructor. Theory and analysis of biochemical catalysis; enzyme kinetics; cofactors; regulation of enzymatic reactions.

B580 Introduction to Biochemical Research (3 cr.) P: graduate standing. Objectives and techniques of biochemical research.

B600 Seminar in Biochemistry (1 cr.) P: B502 or consent of instructor. Advanced critical analysis of the current scientific literature and scientific presentations. Attendance and participation in the weekly Biochemistry Program seminar series is required.

B601 Advanced Nucleic Acid Biochemistry (1.5 cr.) P: B501 or consent of instructor. Mechanistic analysis of nucleic acid metabolism; specificity and role of DNA polymerases and repair pathways; DNA replication and recombination mechanisms; RNA structural motifs and physical properties; RNA synthesis and processing in gene expression; catalytic RNA molecules; applications of RNA molecules.

B602 Advanced Protein Biosynthesis and Processing (1.5 cr.) P: B501 or consent of instructor. Detailed analysis of protein synthesis, post-translational modification, and macromolecular assembly, including the role these modifications play in mature protein function; byosynthesis, structure, function, and analysis of complex oligosaccharides.

B603 Advanced Macromolecular Structure and Interactions (1.5 cr.) P: B503 or consent of instructor. Supplements and extends B503: emphasis on stability and folding mechanisms of proteins and nucleic acids and detailed thermodynamic analysis of binding interactions.

B604 Structural Methods (1.5 cr.) P: B503 or consent of instructor. Fundamental principles of circular dichroism, nuclear magnetic resonance and X-ray crystallography in the study of protein and nucleic acid structures. Theoretical and practical aspects will be presented, with particular emphasis on application strategies.

B605 Structure and Function of Biological Membranes (1.5 cr.) P: B501, B503 or consent of instructor. Biochemistry and biophysics of lipids, membranes and membrane proteins; fundamentals of membrane transport; interfacial catalysis; transmembrane signal transduction.

B680 Special Topics in Biochemistry (1.5-3 cr.) P: Consent of instructor. Topics vary yearly and include the following: Physico-chemical techniques in the study of macromolecules; experimental methods in enzymology; organic chemistry of enzymatic reactions and enzyme models; conformational properties and macromolecules. Can be retaken for credit.

B880 Research: Biochemistry (cr. arr.)*

Cross-Listed Courses

Biology

L529 Bioinformatics in Molecular Biology and Genetics: Practical Applications (4 cr.) P: I501, I502, L519 or consent of instructor. Practical experience in a range of data analysis and software engineering methods applied to molecular biology data.

L585 Molecular Genetics (3 cr.) P: L364 and C483 or equivalent. The molecular basis of genetic interactions, with emphasis on microbial systems. The course covers the molecular mechanisms of mutation, suppression, recombination, complementation, etc., as well as mechanisms for gene transfer in bacteria and bacteriophage. The application of genetic analysis to a variety of molecular biological topics is emphasized.

L586 Molecular Analysis of Cell Biology (3 cr.) Critical analysis of recent advances in our understanding of molecular organization of cellular structures and of their mode of function. The primary interest of this course concerns the eukaryotic cell.

M525 Topics in Microbial Biochemistry and Physiology (3 cr.) P: graduate standing and C483 or M350 or equivalent. The course will consider topics in physiology and biochemistry of eukaryotic and prokaryotic microorganisms. Subjects include membrane physiology and regulatory networks in metabolism and gene expression.

Chemistry

C615 Bioanalytical Chemistry (1.5-3 cr.) P: C511, C512. Survey of modern analytical techniques, including spectrochemical, electrochemical, and separation methods used in biochemical analysis and their applications. (May be given in alternate years).

C632 Structure, Function, and Spectroscopy of Metal Ions in Biological Systems (3 cr.) Introduction to the field of bioinorganic chemistry and spectroscopic methods for determining structure/function relationship of metal ions in biology. Emphasis on oxygen carriers, metal ion transport and storage, as well as oxidoreductases involved in oxygen, hydrogen and nitrogen metabolism. A discussion of electron transfer proteins, photosystems, and the role of metals in medicine will also be included.

Medical Sciences

B801 Molecular and Cellular Biochemistry (3 cr.) P: Graduate standing and consent of instructor. Biochemistry for medical students, emphasizing structure-function relationships of cellular components, biosynthesis of nucleic acids and proteins, degradation of simple and complex cell constituents, and regulation of cell growth.

B802 Metabolism and Signal Transduction (3 cr.) P: Graduate standing and consent of instructor. Biochemistry for medical students, including signaling pathways, membrane biochemistry, and the metabolism of macromolecules in health and disease with emphasis on clinical applications.

Physics

P575 Introductory Biophysics (3 cr.) P: Two out of three from the following: (1) P221/222 and P301 or equivalent, (2) C105/C106 or equivalent, and (3) L221 and L312 or equivalent; or consent of instructor. Overview of cellular components; basic structures of proteins, nucleotides, and biological membranes; solution physics of biological molecules, mechanics and motions of biopolymers; physical chemistry of binding affinity and kinetics; physics of transport and signal transduction; biophysical techniques such as microscopy and spectroscopy; mathematical modeling of biological systems.

Neural Sciences

N612 Ion Channels and Receptors (3 cr.) P: Graduate status and consent of instructor. Molecular, biophysical, and biochemical analysis of the major molecules responsible for neural excitability and synaptic transmission: receptor-coupled ion channels, voltage-dependent ion channels, G-protein coupled receptors, transporters, signal transduction pathways, synaptic vesicle-associated proteins, cytoskeletal proteins, classical and novel neurotransmitters and modulators.

Graduate Faculty
Associate Biochemistry Faculty
Degrees Offered
Special Departmental Requirements
Master of Science Degree
Doctor of Philosophy Degree
Certificate in Biotechnology
Courses

Indianapolis Program

(Department of Biochemistry and Molecular Biology)

School of Medicine

Chairperson
Professor Robert A. Harris

Graduate Advisor
Mark Goebl

Director of Biotechnology Certificate Program
William Bosron

Departmental E-mail
biochem@iupui.edu

Departmental URL
www.biochemistry.iu.edu

Graduate Faculty

Showalter Professor
Robert A. Harris

Distinguished Professor
Robert A. Harris

Professors
David Allmann, Veena B. Antony*, William Bosron, Donald Bowman (Emeritus), Anna DePaoli-Roach, Howard Edenberg, Rose Fife (Medicine), Mark Goebl, Jean Hamilton-Steinrauf, Edwin Harper, Maureen Harrington, Thomas Hurley, Hiremagalur N. Jayaram, Peter Roach, Roger Roeske, Arthur Schulz (Emeritus), Ronald Wek

Associate Professors
Mark A. Deeg, Suk-Hee Lee, Lawrence Quilliam, James P. Walsh

Assistant Professors
Matthew S. Grow*, John W. Hawes*, Debbie C. Thurmond*

Associate Biochemistry Faculty

Distinguished Professor
Ting Kai Li (Medicine)

Professors
W. Marshall Anderson (Northwest Center for Medical Education), Martin Bard (Biology), David Crabb (Medicine), Theodore Gabig (Medicine), Robert Hromas (Medicine), Larry Jones (Medicine), Mark Kelley (Pediatrics), Lawrence Lumeng (Medicine), William McBride Jr. (Neurobiology), Byron Olson (Dentistry), Barth Ragatz (Fort Wayne Center for Medical Education), John Richardson (Bloomington), Christian Maxmillian Schmidt*, Jay Simon (Neurobiology), David Skalnik (Pediatrics), Godfrey Tunnicliff (Evansville Center for Medical Education), Mervin Yoder Jr. (Pediatrics)

Associate Professors
Simon Atkinson (Medicine), Dring Crowell (Biology), David Daleke (Bloomington), Donald L. Durden, Shao-Ling Fong (Ophthalmology), Michael W. King (Terre Haute Center for Medical Education), Edward McKee (South Bend Center for Medical Education), David Potter* (Medicine), Stephen Randall (Biology), Kent Redman* (Fort Wayne Center for Medical Education), Simon Rhodes* (Biology), James Walsh* (Medicine), Theodore Widlanski (Bloomington)

Adjunct Associate Professors
Robert A. Dean* (Pathology), Mark Deeg* (Medicine), Alexander V. Skurat*, Thomas Stephens (Affiliate Graduate Faculty Status), Terry Vik* (Medicine)

Assistant Professors
Kristin Chun* (Pediatrics), Joseph Dynlacht* (Radiation Oncology), Jeffrey S. Elmendorf* (Physiology), Harikrishna Nakshatri* (Medicine), Weinian Shou* (Physiology), Claire E. Walczak* (Bloomington)

Associate Scientist
John W. Hawes*

Assistant Scientist
Dan Spandau* (Medicine)

Adjunct Assistant Professors
Ronald R. Bowsher (Affiliate Graduate Faculty Status), David Timm*

Graduate Advisor
Professor Mark Goebl

Director of Biotechnology Certificate Program
William Bosron

Degrees Offered

Master of Science and Doctor of Philosophy

Special Departmental Requirements

(See also general University Graduate School requirements and departmental brochure.)

Admission Requirements
Typically, a baccalaureate degree in biology, chemistry, or physics that includes calculus and organic chemistry is required for admission. The General Test of the Graduate Record Examination is required.

Master of Science Degree

Course Requirements
A minimum of 30 credit hours, including the core curriculum courses B807, B810, G817, and G865 or any three core courses plus G841, G890 or G910; and at least 6 credit hours, but not more than 9 credit hours, in research. Participation in student seminar B890 is required. G504 Introduction to Research Ethics is also required.

Final Examination
Oral, covering thesis and course work.

Doctor of Philosophy Degree

Course Requirements
A total of 90 credit hours, of which a minimum of 32 credit hours must be in courses other than research, including the core curriculum courses B807, B810, G817, and G865. Participation in student seminar B890 is required every semester of residence. G504 Introduction to Research Ethics is also required.

Grades
A minimum grade point average of 3.0 (B) must be maintained in all nonresearch course work.

Minor
A minimum of 12 credit hours in one of the following programs: life science, physical science, anatomy, biophysics, cancer biology, chemistry, diabetes, medical genetics, microbiology, neurobiology, pathology, pharmacology, physics, physiology, or toxicology.

Qualifying Examinations
Students meet once every six months with their advisory committee to review progress in course work and the dissertation research proposal. Usually, at the completion of the second semester of study, students sit for a written qualifying examination. The final examination in the series is an oral defense of a written research proposal. The nature of the examination over the minor is determined by the member of the advisory committee representing that area. It may consist of a separate examination but is usually part of the written and oral examinations. Continuation of a student in the program depends upon satisfactory performance and progress in each phase of the program.

Dissertation
A minimum of 45 credit hours in research, completed with a grade point average of 3.0 (B) or above. It is expected that the dissertation will qualify for publication in a recognized journal.

Final Examination
Oral, covering dissertation, major, and minor.

Minor in Molecular Biology
See entry under Microbiology and Immunology.

Minor in Life Science
A minimum of 12 credit hours outside the student's major department, chosen from the biological sciences or from the following departments: anatomy, biochemistry, biophysics, dental sciences, medical genetics, microbiology, pathology, pharmacology, physiology, toxicology. At least 6 credit hours must be taken in one of the listed departments or in the biological sciences. The minor program must be approved by the student's advisory committee, the minor representative on which must be selected from one of the departments in which courses for the minor are taken.

Minor in Cancer Biology
Cancer Biology Training Program (CBTP) faculty are members of the Indiana University Cancer Center, the matrix organization for an extensive range of cancer efforts and activities. Ongoing NIH- and ACS-funded research programs focus on Regulation of Cell Growth, Hematopoiesis, Experimental Therapeutics, Adult Oncology, and Pediatric Oncology. CBTP students will fulfill the requirements of their individual basic science departments and complete the cancer biology minor.

Minor in Diabetes
Preceptors with diabetes-related projects are selected from the basic Science Department graduate programs or interdisciplinary programs. Students will fulfill the requirements of the respective program and additionally will complete a Minor in Diabetes and Obesity.

Minor in Diabetes and Obesity
A minimum of 12 credit hours outside of the student's major department including G805 Diabetes and Obesity. At least one credit of G504 Introduction to Research Ethics must also be taken. Other courses are selected from the following list:

B800 Medical Biochemistry (3 cr.)
B807 Protein Structure and Function (3 cr.)
B810 Cellular Biochemistry and Regulation (3 cr.)
C603 General Pathology (6 cr.)
G817 Cell Biology (2 cr.)
G818 Cellular and Integrative Physiology (3 cr.)
G706 Cell-Cell Communication (3 cr.)
G865 Fundamental Molecular Biology (3 cr.)
G910 Advanced Molecular Biology Methods (3 cr.)
G804 Cellular and Molecular Biology (3 cr.)
J840 Mechanisms of Immune Regulation (3 cr.)
J805 Molecular Immunology (3 cr.)
J807 Seminar in Immunology (2 cr.)
F598 Drugs, Diseases and Poisons (3 cr.)
F804 Introduction to Pharmacology and Toxicology I (3 cr.)
F814 Introduction to Pharmacology and Toxicology II (3 cr.)
F809 Neuropharmacology (3 cr.)
F810 Pharmacology of Autonomic Cardiovascular Control (3 cr.)
Q580 Basic Human Genetics (3 cr.)
BIOL564 Molecular Genetics of Development (3 cr.)

The minor program must be approved by the student's Advisory Committee, which will take into consideration the student's total didactic experience. In the case of combined MD/PhD students, the Committee may approve substitution of appropriate medical school courses for the electives. The minor representative will be selected from outside the student's major department and must be approved by the Diabetes and Obesity Training Program.

Certificate in Biotechnology

Admission Requirements
A baccalaureate degree in a scientific field with a minimum science GPA of 3.0/4.0. Proficiency in English by obtaining a degree from a certified American University or a minimum score of 550 on the TOEFL.

Specific Requirements
A fixed curriculum of 15 credits. This will include one introductory course in biochemistry, such as B500 Introductory Biochemistry. This course should be completed before enrolling in the laboratory courses. If a student has taken a a recent biochemistry survey course, the student must substitute an advanced graduate lecture course that is relevant to biotechnology. Such courses may include: G865 Fundamental Molecular Biology: B807 Protein Structure and Function; G817 Eukaryotic Cell Biology, G910 Advanced Molecular Biology Methods, K540 Topics in Biotechnology, or C636 Biochemistry Structural Aspects. Students may take the remaining courses in any order. Three laboratory courses in biotechnology: G841 Methods in Protein Chemistry (3 credits), G890 Methods in Molecular Biology and Pathology (3 credits), G823 Methods in Cellular Metabolism (3 credits). An ethics course: G504 Introduction to Research Ethics (1 credit). Two semesters of a problem-based learning course in biotechnology: G818 Concepts in Biotechnology (1 credit each). Students will be required to maintain a B average and have no less than a grade of B- in each of the required courses for the 15 credit certificate. The maximum time allowed for completion of the program will be three years because of the rapid changes characteristic of the field.

Advisory Committee
The program and student advisory committee will include the program director, directors of the core courses, laboratory director for the program, and one or more representatives from a biotechnology-related company.

Competency Requirements
In the laboratory and problem-based learning courses, students will be required to demonstrate basic competencies necessary for success as a researcher in industry or academia. These competencies include general skills in communication, problem-solving and lifelong learning, as well as specific biotechnology skills in good laboratory conduct, laboratory units of measure, computational and statistical analysis, and biotechnology instrumentation.

Courses

B500 Introductory Biochemistry (3 cr.) P: C341 or equivalent. Structures of carbohydrates, proteins, lipids, and nucleic acids. Basic principles of enzyme catalysis, protein synthesis, intermediary metabolism, and nutrition.

B800 Medical Biochemistry (3 cr.) P: one semester of organic chemistry. Structure and function of biological molecules, regulation of cellular processes by nutrients and hormones, biochemical and molecular basis of disease.

B803 Advanced Biochemistry (cr. arr., max. of 3 cr.) Tutorial instruction in biochemistry.

B805 Diabetes and Obesity (3 cr.) P: one semester of biochemistry. Biochemistry, cell biology, molecular biology, genetics, immunology, and pathophysiology of diabetes and obesity. Topics include metabolic regulation, signal transduction, insulin resistance, insulin production, beta-cell function, animal models, complications, nutrition, prevention, and therapy.

B807 Protein Structure and Function (3 cr.) P: two semesters of organic chemistry; one semester of biochemistry. Physical forces stabilizing protein structure; protein folding. Essential features of macromolecular interactions. Introduction to enzyme kinetics and chemical mechanism in enzyme reactions.

B808 Physical Biochemistry (3 cr.) P: two semesters of physical chemistry; two semesters of calculus; one semester of biochemistry. Thermodynamics and biophysical chemistry of protein, enzymes, nucleic acids, and membranes.

B809 Advanced Organic Chemistry (1-3 cr.) P: two semesters of organic chemistry; two semesters of physical chemistry; B807 or consent of instructor. Tutorial instruction in organic chemistry, as applied to biochemistry.

B810 Cellular Biochemistry and Regulation (3 cr.) P: two semesters of organic chemistry; one semester of biochemistry. Fundamental pathways of metabolism, with emphasis on the mechanisms of metabolic regulation. Mechanisms of signal transduction and the control of cellular function by hormones, growth factors, and other extracellular regulators.

B811 Advanced Intermediary Metabolism (1-3 cr.) P: B810. Tutorial instruction in specialized areas of metabolism.

B813 Chemistry of Steroids (3 cr.) P: two semesters of organic chemistry. Isolation, structure, and determination. Chemical properties and synthesis of the steroids.

B814 Advanced Enzymology (1-3 cr.) P: B807 or B810. Tutorial instruction in enzyme isolation and kinetics.

B830 Biochemical Nutrition (3 cr.) P: two semesters of organic chemistry; one semester of biochemistry. Metabolic utilization of biological fuels. Components required in animal nutrition; essential amino and fatty acids, vitamins, and trace elements. Biochemical derangements in nutritional deficiencies, metabolic disease states, and starvation.

B835 Neurochemistry (3 cr.) P: two semesters of organic chemistry; one semester of biochemistry, or consent of instructor. Metabolism of nervous system tissue. Neurochemical techniques.

B836 Advanced Topics in Neurochemistry (2 cr.) P: B835 or equivalent. Selected topics in neurochemistry dealing with specialized functions of the nervous system.

B842 Instrumentation and Methods of Analysis II (3 cr.) P: two semesters of organic chemistry; one semester of biochemistry.

B848 Mathematics for Biochemistry (3 cr.) Differential equations and topics in advanced calculus.

B854 Introduction to Research (1 cr.) P: two semesters of organic chemistry; two semesters of physical chemistry, one semester of biochemistry, or consent of instructors. Tutorial and laboratory instruction in biochemistry. Purpose is to introduce students in biochemistry to three different research programs.

B855 Research (cr. arr.)

B857 Biochemistry of Exercise (3 cr.) P: B800, B810, or consent of instructors. Study of the biochemical and physiological changes in the human body due to exercise; consequences of long-term exercise programs.

B858 Physical Chemistry (3 cr.) P: one year of physical chemistry. Quantum chemistry and molecular spectra. Statistical mechanics and thermodynamics. Kinetics. Liquid state.

B859 Advanced Physical Chemistry (1-3 cr.) P: B808 or B858. Tutorial instruction in physical chemistry as an extension of either B808 or B858.

B860 Biophysical Protein Chemistry (3 cr.) P: two semesters of physical chemistry; two semesters of calculus. Physical chemistry of particular interest to the biologist. Chemical and physical properties of protein. Multiple equilibria, radiation interactions, transport phenomena.

B868 Advanced Molecular Biology (1-3 cr.) P: G865 or equivalent. Tutorial instruction in specialized area of molecular biology.

B890 Seminar (1 cr.)

G804 Cellular and Molecular Biology (3 cr.) P: one semester of organic chemistry. Cellular and molecular biology that emphasizes the structural organization, biochemistry and molecular biology of cells. Includes cellular processes, development, and differentiation and their relationship to medicine.

G805 Diabetes and Obesity (3 cr.) P: one semester of biochemistry. Biochemistry, cell biology, molecular biology, genetics, immunology and pathophysiology of diabetes and obesity. Topics include metabolic regulation, signal transduction, insulin resistance, insulin production, beta-cell function, animal models, complications, nutrition, prevention and therapy.

G817 Eukaryotic Cell Biology (2 cr.) P: one semester of biochemistry. Organization and function of subcellular structures. Intracellular coordination of cell activity: protein and RNA trafficking, chromatin dynamics, and intracellular processing of receptor mediated signals.

G818 Concepts in Biotechnology (1 cr.) P: B500 or equivalent. Case studies exploring topics on the cutting-edge of biotechnology and tutorials in biotechnology calculations.

G823 Concepts in Biotechnology (1 cr.) P: B500 or equivalent. Discussion and laboratory instruction in modern methods for cell culture, microscopy, flow cytometry and the use of cell culture to study cellular metabolism.

G841 Methods of Protein Chemistry (3 cr.) P: B500 or equivalent. Discussion and laboratory instruction in modern methods for protein purification, analysis of purity, peptide mapping, and amino acid sequencing.

G865 Fundamental Molecular Biology (3 cr.) P: B800 or equivalent. Principles of molecular structure, function, and biosynthesis; core information regarding procaryotic and eukaryotic gene continuity and metabolic coordination; introduction to multicellular systems and problems. (Joint program: biochemistry, medical genetics, microbiology.)

G890 Methods in Molecular Biology and Pathology (3 cr.) P: G865 and/or J838, and consent of instructor. Basic principles and techniques in molecular biology and pathology. Particular emphasis will be on molecular techniques that can be used to study problems related to biochemistry and pathology.

G910 Advanced Molecular Biology Methods (3 cr.) P: G865 and/or G890 and consent of instructor. Advanced theory and techniques in molecular biology. The focus of the course will be on techniques related to manipulation of cloned DNA to study their expression, structure and function.